Journal article

Monocytes From Children With Clinically Stable Cystic Fibrosis Show Enhanced Expression of Toll-Like Receptor 4

Nina C Sturges, Matthew E Wikstroem, Kaye R Winfield, Samantha E Gard, Siobhain Brennan, Peter D Sly, John W Upham



SUMMARY: Lung disease in patients with cystic fibrosis (CF) is characterized by recurrent bacterial respiratory infections and intense airway inflammation. Pattern recognition receptors such as Toll-like receptor 2 (TLR2) and TLR4 identify bacterial pathogens and activate the innate immune response. We therefore hypothesized that increased expression of these receptors would be found on circulating immune cells from children with CF. A cohort of 66 young children (median age 3 years) with CF was studied and compared to both healthy controls (n = 14) and children without CF who were being investigated for recurrent respiratory infections (non-CF disease controls; n = 17) of a similar age. Sur..

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Funding Acknowledgements

The authors thank the children and their families who participated in this study and staff at the Department of Respiratory Medicine at Princess Margaret Hospital involved in the collection and processing of samples. Thanks are given to Helen Palmer for her assistance in setting up techniques. This work was supported by the Australian Cystic Fibrosis Research Trust, the Cystic Fibrosis Foundation (USA), and the National Health and Medical Research Council (Australia).