Journal article
Modulation of bone morphogenic protein signalling alters numbers of astrocytes and oligodendroglia in the subventricular zone during cuprizone-induced demyelination
HS Cate, JK Sabo, D Merlo, D Kemper, TD Aumann, J Robinson, TD Merson, B Emery, VM Perreau, TJ Kilpatrick
Journal of Neurochemistry | Published : 2010
Abstract
The adult subventricular zone (SVZ) is a potential source of precursor cells to replace neural cells lost during demyelination. To better understand the molecular events that regulate neural precursor cell responsiveness in this context we undertook a microarray and quantitative PCR based analysis of genes expressed within the SVZ during cuprizone-induced demyelination. We identified an up-regulation of the genes encoding bone morphogenic protein 4 (BMP4) and its receptors. Immunohistochemistry confirmed an increase in BMP4 protein levels and also showed an increase in phosphorylated SMAD 1/5/8, a key component of BMP4 signalling, during demyelination. In vitro analysis revealed that neural ..
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Funding Acknowledgements
This work was supported by the National Health and Medical Research Council of Australia (NHMRC) and the Holloway Family Trust. We also acknowledge the generous support from Bayer Schering Pharma. We thank Faye Doherty, Melissa Klein, Leonie Cluse and Catherine Chang for technical support. H. S. C. was supported by a NHMRC Peter Doherty Fellowship and a William Collie Fellowship, University of Melbourne. J.K.S. was supported by a Multiple Sclerosis Research Australia (MSRA) Postgraduate Scholarship. B. E. was supported by a NHMRC CJ Martin Fellowship. T. D. M. was supported by a NHMRC/MSRA Betty Cuthbert Fellowship.