Cross-fostering and improved lactation ameliorates deficits in endocrine pancreatic morphology in growth-restricted adult male rat offspring
AL Siebel, LA Gallo, TC Guan, JA Owens, ME Wlodek
JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE | CAMBRIDGE UNIV PRESS | Published : 2010
Uteroplacental insufficiency and poor postnatal nutrition impair adult glucose tolerance and insulin secretion in male rat offspring, which can be partially ameliorated by improving postnatal nutrition. Uteroplacental insufficiency was induced in the WKY rat on day 18 of pregnancy (Restricted) compared to sham-operated Controls. Pups were then cross-fostered onto Control or Restricted mothers one day after birth resulting in: (Pup-on-Mother) Control-on-Control, Control-on-Restricted, Restricted-on-Control and Restricted-on-Restricted. Endocrine pancreatic morphology and markers of intrinsic β-cell function and glucose homeostasis were assessed in male offspring at 6 months. Pancreatic and he..View full abstract
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Awarded by National Health and Medical Research Council of Australia (NHMRC)
The authors wish to thank Mr Chris Chiu and Dr Miles De Blasio for technical assistance with real-time PCR and COBAS analysis, respectively. The authors also thank Kerryn Westcott for professional assistance with animal surgery and handling. ALS present contact address: Baker IDI Heart & Diabetes Institute, Victoria, Australia. This research was supported by a grant from the National Health and Medical Research Council of Australia to MEW (NHMRC; no. 400004), The University of Melbourne ECR grant to ALS and ANZ trusts grant to ALS. ALS was supported by an NHMRC Peter Doherty Biomedical Research Fellowship.