Pharmacological profiles of the murine gastric and colonic H,K-ATPases
Jiahong Shao, Michelle L Gumz, Brian D Cain, Shen-Ling Xia, Gary E Shull, Ian R van Driel, Charles S Wingo
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | ELSEVIER SCIENCE BV | Published : 2010
BACKGROUND: The H,K-ATPase, consisting of α and ß subunits, belongs to the P-type ATPase family. There are two isoforms of the α subunit, HKα₁ and HKα₂ encoded by different genes. The ouabain-resistant gastric HKα₁-H,K-ATPase is Sch28080-sensitive. However, the colonic HKα₂-H,K-ATPase from different species shows poor primary structure conservation of the HKα₂ subunit between species and diverse pharmacological sensitivity to ouabain and Sch28080. This study sought to determine the contribution of each gene to functional activity and its pharmacological profile using mouse models with targeted disruption of HKα₁, HKα₂, or HKbeta genes. METHODS: Membrane vesicles from gastric mucosa and dista..View full abstract
Awarded by National Institutes of Health
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
The authors wish to thank Alicia Rudin and Jeannette Lynch for their assistance in the animal studies. This work was supported by the Department of Veterans Affairs and the National Institutes of Health Grant DK-49750.