Journal article
Characterization of NaV1.6-mediated Na currents in smooth muscle cells isolated from mouse vas deferens
HL Zhu, A Shibata, T Inai, M Nomura, Y Shibata, JA Brock, N Teramoto
Journal of Cellular Physiology | WILEY-BLACKWELL | Published : 2010
DOI: 10.1002/jcp.22032
Abstract
Patch-clamp experiments were performed to investigate the behavior of voltage-activated inward currents in vas deferens myocytes from Na V1.6-null mice (NaV1.6-/-) lacking the expression of the Na+ channel gene, Scn8a, and their wild-type littermates (NaV1.6+/+). Immunohistochemistry confirmed expression of NaV1.6 in the muscle of NaV1.6 +/+, but not NaV1.6-/-, vas deferens. PCR analysis revealed that the only β1-subunit gene expressed in NaV1.6+/+ vas deferens was Scn / b. In Na V1.6+/+ myocytes, the threshold for membrane currents evoked by 20 msec voltage ramps (-100mV to 60 mV) was -38.5±4.6mV and this was shifted to a more positive potential (-31.2±4.9 mV) by tetrodotoxin (TTX). In NaV1..
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Grants
Awarded by Japanese Society for the Promotion of Science (JSPS)
Awarded by Scientist Exchange Program between JSPS and the Australian Academy of Science
Awarded by National Health and Medical Research Council of Australia
Awarded by Grants-in-Aid for Scientific Research
Funding Acknowledgements
Contract grant sponsor: Japanese Society for the Promotion of Science (JSPS); Grant-in-Aid for Scientific Research (B); Contract grant number: 20300178; Contract grant sponsor: JSPS, Grant-in-Aid for Exploratory Research; Contract grant number: 21650129; Contract grant sponsor: JSPS, Grant-in-Aid for Young Scientist (B) Contract grant number: 20710157; Contract grant sponsor: JSPS, Grant-in-Aid for Young Scientist (Start-up); Contract grant number: 21890175. Contract grant sponsor: Japan China Medical Association. Contract grant sponsor: Scientist Exchange Program between JSPS and the Australian Academy of Science; Contract grant number: 2008-AAS. Contract grant sponsor: National Health and Medical Research Council of Australia senior research fellowship; Contract grant number: 350904.