Journal article

c-Jun N-terminal kinase/c-Jun inhibits fibroblast proliferation by negatively regulating the levels of stathmin/oncoprotein 18

Yvonne YC Yeap, Ivan HW Ng, Bahareh Badrian, Tuong-Vi Nguyen, Yan Y Yip, Amardeep S Dhillon, Steven E Mutsaers, John Silke, Marie A Bogoyevitch, Dominic CH Ng

BIOCHEMICAL JOURNAL | PORTLAND PRESS LTD | Published : 2010

Abstract

The JNKs (c-Jun N-terminal kinases) are stress-activated serine/threonine kinases that can regulate both cell death and cell proliferation. We have developed a cell system to control JNK re-expression at physiological levels in JNK1/2-null MEFs (murine embryonic fibroblasts). JNK re-expression restored basal and stress-activated phosphorylation of the c-Jun transcription factor and attenuated cellular proliferation with increased cells in G1/S-phase of the cell cycle. To explore JNK actions to regulate cell proliferation, we evaluated a role for the cytosolic protein, STMN (stathmin)/Op18 (oncoprotein 18). STMN, up-regulated in a range of cancer types, plays a crucial role in the control of ..

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