Characterization of a Caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from Haemonchus contortus (Nematoda)
Bronwyn E Campbell, Elida M Rabelo, Andreas Hofmann, Min Hu, Robin B Gasser
MOLECULAR AND CELLULAR PROBES | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2010
A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larval stages or eggs. The full-length gene (2854 bp) contained ten exons and nine introns, and encoded a cDNA of 951 bp. Comparisons of the conceptually translated protein (316 amino acids, estimated at approximately 35 kDa) with serine/threonine phosphatases (STPs) from other organisms revealed the presence of the conserved motif LRGNHE. Structural analysis, by comparative modellin..View full abstract
Funding from the Australian Research Council is gratefully acknowledged. WormBase WS210 was used in the present study.