A vacuolar-type proton (H ) translocating ATPase alpha subunit encoded by the Hc-vha-6 gene of Haemonchus contortus
Min Hu, Li He, Bronwyn E Campbell, Weiwei Zhong, Paul W Sternberg, Robin B Gasser
MOLECULAR AND CELLULAR PROBES | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2010
In the present study, a full-length cDNA (designated Hc-vha-6) inferred to encode an alpha subunit of a vacuolar-type proton translocating adenosine triphosphatase (V-ATPase) was isolated from the parasitic nematode Haemonchus contortus, and characterized. The transcript for Hc-vha-6 was detected in all developmental stages and both sexes of H. contortus. Elements, including two TATA box (TATAA), two inverted CAAT box (ATTGG), five E box (CANNTG) and six GATA as well as five inverse GATA (TTATC) transcription factor motifs, were identified in the non-coding region upstream of Hc-vha-6. The open reading frame (ORF) of 2601 nucleotides encoded a protein (Hc-VHA-6) of 866 amino acids and a mole..View full abstract
Funding from the Australian Research Council, the Australian Academy of Science, the Australian-American Fulbright Commission are gratefully acknowledged. PWS is an Investigator with the Howard Hughes Medical Institute.