The interaction of sonochemically synthesized gold nanoparticles with serum albumins
Selvaraj Naveenraj, Sambandam Anandan, Arunkumar Kathiravan, Rajalingam Renganathan, Muthupandian Ashokkumar
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | ELSEVIER | Published : 2010
Spherical gold nanoparticles of approximately 16nm were synthesized using a sonochemical reduction method and characterized using UV-vis spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM). The binding of these gold nanoparticles with bovine serum albumin (BSA) and human serum albumin (HSA) was investigated using UV-vis absorption and fluorescence spectroscopic techniques. A strong quenching of the fluorescence from serum albumins was observed due to the formation of a ground state complex with gold nanoparticles (static quenching). The fluorescence quenching constants, number of binding sites and binding constants were determined using Stern-Volmer and Ben..View full abstract
Awarded by DST, New Delhi
Authors SA and MA thank DST, New Delhi (INT/AUS/P-1/07 dated 19th Sep 2007) and DEST, Australia for the sanction of INDIA-AUSTRALIAN strategic research fund for their collaborative research. Also the authors thank Prof. P. Ramamurthy, Director, National Centre for Ultra Fast Processes, University of Madras, Taramani Campus, Chennai, India for analysing lifetime measurements.