Unique biochemical properties of the protein tyrosine phosphatase activity of PTEN-Demonstration of different active site structural requirements for phosphopeptide and phospholipid phosphatase activities of PTEN
Joel Yeong-Chit Chia, Joanna E Gajewski, Yi Xiao, Hong-Jian Zhu, Heung-Chin Cheng
Biochimica et Biophysica Acta-Proteins and Proteomics | ELSEVIER SCIENCE BV | Published : 2010
The work described in this manuscript is supported by project grants from the National Health and Medical Research Council of Australia (to H.-C. Cheng) and Cancer Council of Victoria project grant (to H.-C. Cheng and H.-J. Zhu). We wish to thank Prof. Bart Vanhaesebroeck of the Institute of Cancer, Batts and The London School of Medicine and Dentistry for his generous gift of the recombinant baculovirus directing the expression of phosphatidylinositide-3 kinase in insect cells. We wish to thank Miss Boonyarin Jarasrassamee for supplying us with her purified recombinant Lyn kinase in this study. We would like to thank Mrs. Daisy Lio for her help in generating the recombinant baculoviruses of PTEN and its mutants as well as her effort in purifying the recombinant PTEN and its mutants. Y.-C. Chia is a recipient of the Australian Endeavour Postgraduate Award.