Coinfection of Hepatic Cell Lines with Human Immunodeficiency Virus and Hepatitis B Virus Leads to an Increase in Intracellular Hepatitis B Surface Antigen
David M Iser, Nadia Warner, Peter A Revill, Ajantha Solomon, Fiona Wightman, Suha Saleh, Megan Crane, Paul U Cameron, Scott Bowden, Tin Nguyen, Candida F Pereira, Paul V Desmond, Stephen A Locarnini, Sharon R Lewin
JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2010
Liver-related mortality is increased in the setting of HIV-hepatitis B virus (HBV) coinfection. However, interactions between HIV and HBV to explain this observation have not been described. We hypothesized that HIV infection of hepatocytes directly affects the life cycle of HBV. We infected human hepatic cell lines expressing HBV (Hep3B and AD38 cells) or not expressing HBV (Huh7, HepG2, and AD43 cells) with laboratory strains of HIV (NL4-3 and AD8), as well as a vesicular stomatitis virus (VSV)-pseudotyped HIV expressing enhanced green fluorescent protein (EGFP). Following HIV infection with NL4-3 or AD8 in hepatic cell lines, we observed a significant increase in HIV reverse transcriptase..View full abstract
This work was supported by a Postgraduate Scholarship from the National Health and Medical Research Council (NHMRC) of Australia (D. M. I.). S. R. L. is an NHMRC Practitioner Fellow.