Journal article

Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis

Eline Thomson, Siobhain Brennan, Revathy Senthilmohan, Catherine L Gangell, Anna L Chapman, Peter D Sly, Anthony J Kettle



We aimed to determine whether myeloperoxidase (MPO) is the main peroxidase present in the airways of children with cystic fibrosis (CF) and to assess which oxidants it produces and whether they are associated with clinical features of CF. Children with CF (n=54) and without CF (n=16) underwent bronchoscopy and bronchoalveolar lavage (BAL) for assessment of pulmonary infection and inflammation. BAL fluid was analyzed for MPO, halogenated tyrosines as markers of hypohalous acids, thiocyanate, and protein carbonyls. MPO was the only peroxidase detected in BAL samples from children with CF and its concentration was markedly higher than in controls. Levels of 3-chlorotyrosine and 3-bromotyrosine ..

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Funding Acknowledgements

We thank the Health Research Council of New Zealand and the Australian Cystic Fibrosis Research Trust for supporting this work. Eline Thomson was supported by the Christchurch School of Medicine and Health Sciences on a McGee Scholarship. Dr. Kettle has received speaking honoraria from AstraZeneca and Lilly and has received research grant support from AstraZeneca. The full authorship of this article includes the members of AREST CF: Elizabeth Balding, Luke J. Berry, Dr. Siobhain Brennan, Professor John B. Carlin, Rosemary Carzino, Professor Nick de Klerk, Dr. Tonia Douglas, Clara Foo, Dr. Catherine L Gangell, Luke W. Garratt, A/Professor Graham L Hall, Dr. Jo Harrison, Dr. Anthony Kicic, Dr. Ingrid A. Laing, Karla M. Logie, A/Professor John Massie, Dr. Lauren S. Mott, Dr. Conor Murray, Faith Parsons, Dr. Naveen Pillarisetti, Dr. Srinivas R. Poreddy, Dr. Sarath C. Ranganathan, Professor Colin F. Robertson, Professor Roy Robins-Browne, A/Professor Philip J. Robinson, Billy Skoric, Professor Peter D. Sly, Professor Stephen M. Stick, Dr. Erika N. Sutanto, and Dr. Elizabeth Williamson.