Cystatin A protease inhibitor and cysteine proteases in human cervicovaginal fluid in term pregnancy and labor
Yujing J Heng, Megan KW Di Quinzio, Michael Permezel, Gregory E Rice, Harry M Georgiou
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | MOSBY-ELSEVIER | Published : 2011
OBJECTIVE: The purpose of this study was to investigate the temporal changes in immunoreactive cystatin A and the enzymatic activity of cathepsins B, H, L, and S in human cervicovaginal fluid (CVF) in late pregnancy and spontaneous labor. STUDY DESIGN: CVF was collected weekly (n = 95 women) from 36 weeks gestation until spontaneous term labor. Cystatin A was quantified using enzyme-linked immunosorbent assay. The enzyme activity of cathepsins B, H, L, and S was measured with fluorometric enzyme assay kits. RESULTS: Cystatin A significantly decreased towards (P = .016, 2-way analysis of variance) and during labor (P < .001, 2-way analysis of variance). Enzymatic activity of cathepsins B, H, ..View full abstract
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Awarded by National Health and Medical Research Council of Australia (NHMRC)
Supported by a National Health and Medical Research Council of Australia (NHMRC) Development Grant (454451) and the Medical Research Foundation for Women and Babies. YJ Heng is a recipient of an NHMRC Biomedical (Dora Lush) Postgraduate Research Scholarship (454880).