Increased ATP-binding cassette transporter A1 expression in Alzheimer's disease hippocampal neurons

WS Kim; S Bhatia; DA Elliott; L Agholme; K Kgedal; H McCann; GM Halliday; KJ Barnham; B Garner

Journal article

Increased ATP-binding cassette transporter A1 expression in Alzheimer's disease hippocampal neurons

WS Kim, S Bhatia, DA Elliott, L Agholme, K Kgedal, H McCann, GM Halliday, KJ Barnham, B Garner

Journal of Alzheimer S Disease | Published : 2010

DOI: 10.3233/JAD-2010-100324

Abstract

ATP-binding cassette transporter A1 (ABCA1) reduces amyloid-β burden in transgenic mouse models of Alzheimer's disease (AD). Associations between ABCA1 polymorphisms and AD risk are also established. Little is known regarding the regulation of ABCA1 expression in the brain and how this may be affected by AD. In the present study we assessed ABCA1 mRNA and protein expression in the hippocampus of AD cases compared to controls. ABCA1 was clearly expressed in hippocampal neurons and expression was increased two-to three-fold in AD cases. The increased hippocampal ABCA1 expression was associated with increased APOE and PUMA gene expression, implying an association with neuronal stress. Consisten..

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University of Melbourne Researchers

Kevin Barnham Author

Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)

Awarded by Australian Research Council (ARC)

Funding Acknowledgements

We are very grateful to Prof Colin Masters and Dr Qiao-Xin Li for providing WO2 antibody and to Prof Andy Hill for providing CHO cells stably expressing human A beta PP695. This research was supported by the Australian National Health and Medical Research Council (NHMRC, Grant No. 510148). SB is supported by an Australian Postgraduate Award PhD scholarship. KJB and GMH are supported by Research Fellowships from the NHMRC. BG is supported by a Research Fellowship from the Australian Research Council (ARC Grant No. FT0991986). DAE is supported by a Viertel Postdoctoral Fellowship awarded by Alzheimer's Australia. Australian Brain Donor Programs Prince of Wales Medical Research Institute Tissue Resource Centre is supported by the NHMRC and the NSW Tissue Resource Centre is supported by the University of Sydney, NHMRC, Schizophrenia Research Institute, National Institute of Alcohol Abuse and Alcoholism and NSW Department of Health.