Journal article

The Legionella pneumophila F-box protein Lpp2082 (AnkB) modulates ubiquitination of the host protein parvin B and promotes intracellular replication

M Lomma, D Dervins-Ravault, M Rolando, T Nora, HJ Newton, FM Sansom, T Sahr, L Gomez-Valero, M Jules, EL Hartland, C Buchrieser

CELLULAR MICROBIOLOGY | WILEY | Published : 2010

Abstract

The environmental pathogen Legionella pneumophila encodes three proteins containing F-box domains and additional protein-protein interaction domains, reminiscent of eukaryotic SCF ubiquitin-protein ligases. Here we show that the F-box proteins of L. pneumophila strain Paris are Dot/Icm effectors involved in the accumulation of ubiquitinated proteins associated with the Legionella-containing vacuole. Single, double and triple mutants of the F-box protein encoding genes were impaired in infection of Acanthamoeba castellanii, THP-1 macrophages and human lung epithelial cells. Lpp2082/AnkB was essential for infection of the lungs of A/J mice in vivo, and bound Skp1, the interaction partner of th..

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Grants

Awarded by Network of Excellence 'Europathogenomics'


Awarded by European Commission


Funding Acknowledgements

We would like to specially thank P. Cossart, M. Bonazzi and the members of the Unite Interactions Bacteries Cellules, Institut Pasteur, for the helpful and friendly discussions and for providing several tools and P. Glaser for critical reading of the manuscript. This work received financial support from the Institut Pasteur, the Centre National de la Recherche (CNRS), the Network of Excellence 'Europathogenomics' LSHB-CT-2005-512061 and the Australian National Health and Medical Research Council (NHMRC). M. Lomma is holder of a Marie Curie fellowship financed by the European Commission (INTRAPATH project MEST-CT-2005-020715) co-ordinated by Institut Pasteur and got support from the Fondation pour la Recherche Medicale (FRM), M. Rolando is financed by the ANR-ERA-Net project FUNGEN, L. Gomez-Valero is holder of a Roux postdoctoral research Fellowship financed by the Institut Pasteur and T. Nora is financed by the DIM maladies Infectieuse, Ile de France.