An efficient method for enumerating oral spirochetes using flow cytometry
Rebecca Orth, Neil O'Brien-Simpson, Stuart Dashper, Katrina Walsh, Eric Reynolds
JOURNAL OF MICROBIOLOGICAL METHODS | ELSEVIER SCIENCE BV | Published : 2010
Spirochetes, such as Treponema denticola, are thin walled, helical, motile bacteria. They are notoriously difficult to enumerate due to their thinness and the difficulties associated with culturing them. Here we have developed a modified oral bacterial growth medium (OBGM) that significantly improves the cultivation of T. denticola compared with a previously published growth medium. Three methods for the enumeration of T. denticola, semi-solid growth medium colony-forming unit (CFU) counts, DNA analysis and flow cytometry, are described and compared. Enumeration of T. denticola using the semi-solid agar method resulted in a positive linear relationship with absorbance of the culture (R(2)=0...View full abstract
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Awarded by Australian National Health and Medical Research Council
This work was supported by the Australian National Health and Medical Research Council (project number 454475).