Journal article
Imaging cellular architecture with X-rays
CA Larabell, KA Nugent
Current Opinion in Structural Biology | CURRENT BIOLOGY LTD | Published : 2010
Abstract
X-ray imaging of biological samples is progressing rapidly. In this paper we review the progress to date in high-resolution imaging of cellular architecture. In particular we survey the progress in soft X-ray tomography and argue that the field is coming of age and that important biological insights are starting to emerge. We then review the new ideas based on coherent diffraction. These methods are at a much earlier stage of development but, as they eliminate the need for X-ray optics, have the capacity to provide substantially better spatial resolution than zone plate-based methods. © 2010 Elsevier Ltd.
Grants
Awarded by National Institutes of Health
Funding Acknowledgements
The authors thank Dr Mark Le Gros for his assistance with this manuscript and Dr Maho Uchida for help with preparation of the figures CAL acknowledges the support of the National Center for Research Resources (RR019664) and the National Institute of General Medical Sciences (GM070445) both of the National Institutes of Health and the US Department of Energy Office of Biological and Environmental Research (Contract No DE AC02 05CH11231) KAN acknowledges the support of the Australian Research Council through its Centres of Excellence and Federation Fellowships programs