Journal article

Activation of neuronal SST and SST receptors decreases neurogenic secretion in the guinea-pig jejunum.

JP Foong, LJ Parry, JC Bornstein

Neurogastroenterology and Motility the Official Journal of the European Gastrointestinal Motility Society | WILEY-BLACKWELL | Published : 2010

Abstract

Vasoactive intestinal peptide (VIP) submucosal neurons, the main regulators of gut secretion, display inhibitory postsynaptic potentials mediated by somatostatin (SOM) acting on SST(1) and SST(2) receptors (SSTR(1), SSTR(2)) in the guinea-pig small intestine. We investigated the implications of this for neurally-evoked mucosal secretion. Mucosal-submucosal preparations from guinea-pig jejunum were mounted in Ussing chambers to measure Cl(-) secretion, measured by short circuit current (I(sc)). All drugs were added serosally. Veratridine (1 μmol L(-1)) was used to stimulate neurons and provide a robust secretory response for pharmacological testing.5-hydroxytrptamine (5-HT, 300 nmol L(-1)) wa..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council Australia (NHMRC)


Awarded by Novartis Pharmaceuticals Australia


Funding Acknowledgements

The study was supported by a grant from the National Health and Medical Research Council Australia (NHMRC Grant No. 40053) and an Australian Postgraduate Award (JPP). The compound PKF225-880-AA-1 (SRA880) was kindly provided by Novartis Pharmaceuticals Australia. We thank Dr Kathleen Neal for her invaluable advice on the manuscript.