Journal article

Influenza Epitope-Specific CD8( ) T Cell Avidity, but Not Cytokine Polyfunctionality, Can Be Determined by TCR beta Clonotype

Jessica M Moffat, Andreas Handel, Peter C Doherty, Stephen J Turner, Paul G Thomas, Nicole L La Gruta

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2010

Abstract

Cytokine polyfunctionality has recently emerged as a correlate of effective CTL immunity to viruses and tumors. Although the determinants of polyfunctionality remain unclear, there are published instances of a link between the production of multiple effector molecules and the peptide plus MHC class I molecule avidity of T cell populations. Influenza A virus infection of C57BL/6J mice induces CTL populations specific for multiple viral epitopes, each with varying proportions of monofunctional (IFN-γ(+) only) or polyfunctional (IFN-γ(+)TNF-α(+)IL-2(+)) CTLs. In this study, we probe the link between TCR avidity and polyfunctionality for two dominant influenza epitopes (D(b)NP(366) and D(b)PA(22..

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Grants

Awarded by National Health and Medical Research Project


Awarded by National Health and Medical Research Program


Awarded by National Institutes of Health


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by National Health and Medical Research Project Grants AI454595 (to N.L.L. and P. C. D.) and AI628316 (to N.L.L.), a National Health and Medical Research Program Grant AI567122 (to P. C. D. and S.J.T.), a National Health and Medical Research RD Wright Career Development Award (to N.L.L.), a Pfizer Australia Research Fellowship (to S.J.T.), and National Institutes of Health Grants AI70251 (to P. C. D.), AI065097, AI077714 (to P. G. T.), and AI072193 (to A.H.).