Journal article

Spatio-Temporal Progression of Grey and White Matter Damage Following Contusion Injury in Rat Spinal Cord

C Joakim Ek, Mark D Habgood, Jennifer K Callaway, Ross Dennis, Katarzyna M Dziegielewska, Pia A Johansson, Ann Potter, Benjamin Wheaton, Norman R Saunders

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2010

Abstract

Cellular mechanisms of secondary damage progression following spinal cord injury remain unclear. We have studied the extent of tissue damage from 15 min to 10 weeks after injury using morphological and biochemical estimates of lesion volume and surviving grey and white matter. This has been achieved by semi-quantitative immunocytochemical methods for a range of cellular markers, quantitative counts of white matter axonal profiles in semi-thin sections and semi-quantitative Western blot analysis, together with behavioural tests (BBB scores, ledged beam, random rung horizontal ladder and DigiGait analysis). We have developed a new computer-controlled electronic impactor based on a linear motor..

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Grants

Awarded by Victorian Neurotrauma Initiative


Funding Acknowledgements

This work was supported by the Victorian Neurotrauma Initiative (Grant #DP048). For more information about this research fund, go to http://www.vni.com.au/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.