Journal article
First transcriptomic analysis of the economically important parasitic nematode, Trichostrongylus colubriformis, using a next-generation sequencing approach
C Cantacessi, M Mitreva, BE Campbell, RS Hall, ND Young, AR Jex, S Ranganathan, RB Gasser
Infection Genetics and Evolution | Published : 2010
Abstract
Trichostrongylus colubriformis (Strongylida), a small intestinal nematode of small ruminants, is a major cause of production and economic losses in many countries. The aims of the present study were to define the transcriptome of the adult stage of T. colubriformis, using 454 sequencing technology and bioinformatic analyses, and to predict the main pathways that key groups of molecules are linked to in this nematode. A total of 21,259 contigs were assembled from the sequence data produced from a normalized cDNA library; 7876 of these contigs had known orthologues in the free-living nematode Caenorhabditis elegans, and encoded, amongst others, proteins with 'transthyretin-like' (8.8%), 'RNA r..
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Awarded by National Institutes of Health
Funding Acknowledgements
This research was supported by grants from the National Human Genome Research Institute (NHGRI) and National Institutes of Health (MM) and the Australian Research Council, the Australian Academy of Science and the Australian-American Fulbright Commission (RBG). CC is the grateful recipient of International Postgraduate Research Scholarship (IPRS) from the Australian Government and a fee-remission scholarship through The University of Melbourne as well as the Clunies Ross (2008) and Sue Newton (2009) awards from the School of Veterinary Science of the same university. The authors thank Gareth Hutchinson for providing T. colubriformis, Weiwei Zhong for advice on genetic interactions and the staff of Worm Base for assistance.