Journal article
Genetic classification of Echinococcus granulosus cysts from humans, cattle and camels in Libya using mutation scanning-based analysis of mitochondrial loci
MH Abushhewa, MHS Abushhiwa, MJ Nolan, AR Jex, BE Campbell, A Jabbar, RB Gasser
Molecular and Cellular Probes | Published : 2010
Abstract
We genetically classified Echinococcus granulosus from humans, cattle and camels in Libya utilizing DNA regions (designated p. cox1 and p. nad1) within the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes, respectively. Polymerase chain reaction (PCR)-based single-strand conformation polymorphism (SSCP) analysis of p. cox1 and p. nad1 amplicons derived from genomic DNA samples from individual cysts (n = 176) revealed four distinct electrophoretic profiles for each locus. Direct sequencing of selected amplicons representing each of these profiles defined four different sequence types for each locus, which were present in five different combinations (de..
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Funding Acknowledgements
The Gasser laboratory is presently in receipt of grants from the Australian Research Council (ARC) and Melbourne Water Corporation ARJ is the recipient of a Career Developmental Award (CDA1) from the National Health and Medical Research Council (NHMRC) Mahmud Abushhewa held a scholarship from the United Nations Educational Scientific and Cultural Organization (UNESCO)