Journal article

Neural crest regionalisation for enteric nervous system formation: Implications for Hirschsprung's disease and stem cell therapy

Dongcheng Zhang, Inigo M Brinas, Benjamin J Binder, Kerry A Landman, Donald F Newgreen

Developmental Biology | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2010


Midbrain, hindbrain and vagal neural crest (NC) produced abundant enteric nervous system (ENS) in co-grafted aneural hindgut and midgut, using chick-quail chorio-allantoic membrane grafts, forming complete myenteric and submucosal plexuses. This ability dropped suddenly in cervical and thoracic NC levels, furnishing an incomplete ENS in one or both plexuses. Typically, one plexus was favoured over the other. This deficiency was not caused by lower initial trunk NC number, yet overloading the initial number decreased the deficiency. No qualitative difference in neuronal and glial differentiation between cranial and trunk levels was observed. All levels formed HuC/D+ve, NOS+ve, ChAT+ve, and TH..

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University of Melbourne Researchers


Funding Acknowledgements

The authors are supported by the National Health and Medical Research Council and the Australian Research Council. We thank Thomas Muller and Craig Smith for FABP and SoxE antibodies, respectively, and also, for many antibodies listed in Table 1, the Developmental Studies Hybridoma Bank ( which was developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, IA 52242.