Journal article
Glucose induces pancreatic islet cell apoptosis that requires the BH3-only proteins bim and puma and multi-BH domain protein bax
MD McKenzie, E Jamieson, ES Jansen, CL Scott, DCS Huang, P Bouillet, J Allison, TWH Kay, A Strasser, HE Thomas
Diabetes | AMER DIABETES ASSOC | Published : 2010
DOI: 10.2337/db09-1151
Abstract
OBJECTIVE - High concentrations of circulating glucose are believed to contribute to defective insulin secretion and β-cell function in diabetes and at least some of this effect appears to be caused by glucose-induced β-cell apoptosis. In mammalian cells, apoptotic cell death is controlled by the interplay of proapoptotic and antiapoptotic members of the Bcl-2 family. We investigated the apoptotic pathway induced in mouse pancreatic islet cells after exposure to high concentrations of the reducing sugars ribose and glucose as a model of β-cell death due to long-term metabolic stress. RESEARCH DESIGN AND METHODS - Islets isolated from mice lacking molecules implicated in cell death pathways w..
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Grants
Awarded by National Cancer Institute
Funding Acknowledgements
[ "This work was supported by fellowships and grants from the National Health and Medical Research Council of Australia (NHMRC) (NHMRC program 461221; NHMRC Career Development Awards to H.E.T. and C.L.S.; NHMRC Australia fellowship to A.S.), the National Institutes of Health (CA-043540-18 and CA-80188-6), and a joint special program grant from the Juvenile Diabetes Research Foundation/NHMRC (program 466658).", "No potential conflicts of interest relevant to this article were reported.", "We thank J.M. Adams, S. Cory, A. Villunger, and M. Labow for gene-targeted mice; M. Narita for qRT-PCR primers; G. Siciliano, N. lannarella, A. Gomes, and S. Fynch for animal care; and R. Ayala-Perez, B. Helbert, C. Youngadn, and C. Dobrzelak for technical assistance and genotyping." ]