Journal article

Differentiation of human adipose-derived stem cells into beating cardiomyocytes

Yu Suk Choi, Gregory J Dusting, Samantha Stubbs, Sandeep Arunothayaraj, Xiao Lian Han, Philippe Collas, Wayne A Morrison, Rodney J Dilley

Journal of Cellular and Molecular Medicine | WILEY | Published : 2010

Abstract

Human adipose-derived stem cells (ASCs) may differentiate into cardiomyocytes and this provides a source of donor cells for tissue engineering. In this study, we evaluated cardiomyogenic differentiation protocols using a DNA demethylating agent 5-azacytidine (5-aza), a modified cardiomyogenic medium (MCM), a histone deacetylase inhibitor trichostatin A (TSA) and co-culture with neonatal rat cardiomyocytes. 5-aza treatment reduced both cardiac actin and TropT mRNA expression. Incubation in MCM only slightly increased gene expression (1.5- to 1.9-fold) and the number of cells co-expressing nkx2.5/sarcomeric alpha-actin (27.2% versus 0.2% in control). TSA treatment increased cardiac actin mRNA ..

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