Differentiation of human adipose-derived stem cells into beating cardiomyocytes
Yu Suk Choi, Gregory J Dusting, Samantha Stubbs, Sandeep Arunothayaraj, Xiao Lian Han, Philippe Collas, Wayne A Morrison, Rodney J Dilley
Journal of Cellular and Molecular Medicine | WILEY | Published : 2010
Human adipose-derived stem cells (ASCs) may differentiate into cardiomyocytes and this provides a source of donor cells for tissue engineering. In this study, we evaluated cardiomyogenic differentiation protocols using a DNA demethylating agent 5-azacytidine (5-aza), a modified cardiomyogenic medium (MCM), a histone deacetylase inhibitor trichostatin A (TSA) and co-culture with neonatal rat cardiomyocytes. 5-aza treatment reduced both cardiac actin and TropT mRNA expression. Incubation in MCM only slightly increased gene expression (1.5- to 1.9-fold) and the number of cells co-expressing nkx2.5/sarcomeric alpha-actin (27.2% versus 0.2% in control). TSA treatment increased cardiac actin mRNA ..View full abstract
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Awarded by National Health and Medical Research Council of Australia
Anita L. Sorensen is thanked for expert help with methylated DNA immunoprecipitation. These studies were supported by grants from National Health and Medical Research Council of Australia 400303;509271 and the Cass Foundation.