Journal article
Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster
H Colinet, SF Lee, A Hoffmann
Journal of Experimental Biology | Published : 2010
DOI: 10.1242/jeb.051003
Abstract
To protect cells from the damaging effects of environmental stresses, all organisms possess a universal stress response involving upregulation of heat shock proteins (Hsps). The mechanisms underlying chilling injuries and the subsequent recovery phase are only beginning to be understood in insects. Hsp22 and Hsp23 are both upregulated during the recovery from prolonged chill coma in Drosophila melanogaster. This prompted us to investigate the functional significance of these modulations by testing whether expression of these two small Hsps is necessary for recovery after cold stress. We used the GAL4/UAS system to separately knock down expression of Hsp22 and Hsp23, and assayed three aspects..
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Funding Acknowledgements
We are grateful to Phillip Daborn and Philip Batterham for providing access to the PC2 facility (Melbourne University, Australia), and we thank Steve McKechnie (Monash University, Australia) for assisting in the importation of fly lines. This study was supported by Fonds de la Recherche Scientifique - FNRS, the Australian Research Council via their Discovery and Fellowship schemes, and the Commonwealth Environmental Research Fund. This paper is number BRC189 of the Biodiversity Research Centre.