Journal article
Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis
S Jurado, I Smyth, B van Denderen, N Tenis, A Hammet, K Hewitt, JL Ng, CJ McNees, SV Kozlov, H Oka, M Kobayashi, LA Conlan, TJ Cole, KI Yamamoto, Y Taniguchi, S Takeda, MF Lavin, J Heierhorst
Plos Genetics | Published : 2010
Open access
Abstract
Zn2+-finger proteins comprise one of the largest protein superfamilies with diverse biological functions. The ATM substrate Chk2-interacting Zn2+-finger protein (ASCIZ; also known as ATMIN and ZNF822) was originally linked to functions in the DNA base damage response and has also been proposed to be an essential cofactor of the ATM kinase. Here we show that absence of ASCIZ leads to p53-independent late-embryonic lethality in mice. Asciz-deficient primary fibroblasts exhibit increased sensitivity to DNA base damaging agents MMS and H2O2, but Asciz deletion or knock-down does not affect ATM levels and activation in mouse, chicken, or human cells. Unexpectedly, Asciz-deficient embryos also exh..
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Awarded by Japan Society for the Promotion of Science
Funding Acknowledgements
This work was supported by grants and fellowships from the National Health and Medical Research Council of Australia to JH, IS, AH, TJC, and MFL; by a Monash University Fellowship to IS; by a Cancer Council Victoria Postdoctoral Fellowship to CJM; by a Center of Excellence Grant for Scientific Research to ST from the Ministry of Education, Culture, Sports, Science and Technology, Japan; and by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, of Japan to KiY and MK. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.