Dendritic cells expressing soluble CTLA4Ig prolong antigen-specific skin graft survival
Francesco L Ierino, William Mulley, Natalie Dodge, Yu Qin Li, Effie Mouhtouris, Dale Christiansen, Mauro S Sandrin
Immunology and Cell Biology | NATURE PUBLISHING GROUP | Published : 2010
Dendritic cells (DCs) and CTLA4Ig are important in regulating T-cell responses and therefore represent potential therapeutic tools in transplantation. In this study, CTLA4Ig was expressed in a C57BL/6 murine DC line (JAWS II) by lentiviral transduction and these cells were used to examine T-cell immunomodulatory effects in vitro and in vivo. A lower stimulation index to C57BL/6 was observed with splenocytes from BALB/c mice primed with JAWS II-CTLA4Ig compared with control JAWS II-green fluorescent protein (JAWS II-GFP). Mice primed with JAWS II-CTLA4Ig cells had significantly prolonged antigen-specific C57BL/6 skin graft survival compared with either JAWS II-GFP-primed or naïve mice (median..View full abstract
This work was supported by funds from the National Health and Medical Research Council of Australia (NHMRC).