Journal article
Both CD31 and CD31- naive CD4 T cells are persistent HIV type 1-infected reservoirs in individuals receiving antiretroviral therapy
F Wightman, A Solomon, G Khoury, JA Green, L Gray, PR Gorry, YS Ho, NK Saksena, J Hoy, SM Crowe, PU Cameron, SR Lewin
Journal of Infectious Diseases | Published : 2010
DOI: 10.1086/656721
Abstract
Background. Naive T cell recovery is critical for successful immune reconstitution after antiretroviral therapy (ART), but the relative contribution of CD31+ and CD31- naive T cells to immune reconstitution and viral persistence is unknown. Methods. In a cross-sectional (n = 94) and longitudinal (n = 10) study of human immunodeficiency virus (HIV)-infected patients before and after ART, we examined the ratio of CD31+ to CD31- naive CD4+ T cells. In the longitudinal cohort we then quantified the concentration of HIV-1 DNA in each cell subset and performed single-genome amplification of virus from memory and naive T cells. Results. Patients receiving ART had a higher proportion of CD31+ CD4 + ..
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Funding Acknowledgements
Financial support: S.R.L., FW, A. S., P. U. C., and S. M. C. are supported by a National Health and Medical Research Council (NHMRC) program grant; S. R. L. is an NHMRC Practitioner Fellow and is also supported by the Alfred Research Trust; J.A.G. was supported by a National Health Group Singapore research grant; P. R. G. is an NHMRC R. Douglas Wright Biomedical Research Fellow; and S. M. C. is an NHMRC Principal Research Fellow.