Journal article

TGF-beta 1 prevents up-regulation of the P2X7 receptor by IFN-gamma and LPS in leukemic THP-1 monocytes

Safina Gadeock, Jimmy NSN Tran, Jennifer G Georgiou, Iman Jalilian, Rosanne M Taylor, James S Wiley, Ronald Sluyter

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | ELSEVIER | Published : 2010

Abstract

The P2X7 receptor is an extracellular ATP-gated cation channel critical in inflammation and immunity, and can be up-regulated by IFN-γ and LPS. This study aimed to examine the effect of TGF-β1 on the up-regulation of P2X7 function and expression in leukemic THP-1 monocytes differentiated with IFN-γ and LPS. Cell-surface molecules including P2X7 were examined by immunofluorescence staining. Total P2X7 protein and mRNA was assessed by immunoblotting and RT-PCR respectively. P2X7 function was evaluated by ATP-induced cation dye uptake measurements. Cell-surface P2X7 was present on THP-1 cells differentiated for 3days with IFN-γ and LPS but not on undifferentiated THP-1 cells. ATP induced ethidi..

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