Conservation of copper-transporting P(IB)-type ATPase function
Adam Southon, Nickless Palstra, Nicholas Veldhuis, Ann Gaeth, Charles Robin, Richard Burke, James Camakaris
BIOMETALS | SPRINGER | Published : 2010
Copper-transporting P(IB)-type ATPases are highly conserved, and while unicellular eukaryotes and invertebrates have only one, a gene duplication has occurred during vertebrate evolution. Copper-induced trafficking of mammalian ATP7A and ATP7B from the trans-Golgi Network towards the plasma membrane is critical for their role in copper homeostasis. In polarized epithelial cells ATP7A and ATP7B traffic towards the basolateral and apical membranes respectively. We examined the localization and function of DmATP7, the single Drosophila melanogaster orthologue, in cultured D. melanogaster and mammalian cells to explore the conservation of P(IB)-type ATPase function. Comparative genomic analysis ..View full abstract
We gratefully acknowledge Dr. Sharon La Fontaine for providing the GM2069 and Me32a cell lines, Dr. Bi-Xia Ke for the pcDNA-hTyr construct and Prof. Paul Gleeson for the GCC88 antibody. We thank Mark Greenough for constructive comments. The Australian Research Council and the Australian Institute of Nuclear Science and Engineering provided grants. The J.C Rowden White Trust provided a grant for partial funding of the Olympus FluoView 1000 confocal microscope. Adam Southon is the recipient of a PhD scholarship from the National Health and Medical Research Council of Australia and the 2009 Dawson Bursary.