Journal article
A small synthetic cripto blocking peptide improves neural induction, dopaminergic differentiation, and functional integration of mouse embryonic stem cells in a rat model of Parkinson's disease
E Lonardo, CL Parish, S Ponticelli, D Marasco, D Ribeiro, M Ruvo, S De Falco, E Arenas, G Minchiotti
Stem Cells | WILEY | Published : 2010
DOI: 10.1002/stem.458
Abstract
Cripto is a glycosylphosphatidylinositol-anchored coreceptor that binds Nodal and the activin type I (ALK)-4 receptor, and is involved in cardiac differentiation of mouse embryonic stem cells (mESCs). Interestingly, genetic ablation of cripto results in increased neuralization and midbrain dopaminergic (DA) differentiation of mESCs, as well as improved DA cell replacement therapy (CRT) in a model of Parkinson's disease (PD). In this study, we developed a Cripto specific blocking tool that would mimic the deletion of cripto, but could be easily applied to embryonic stem cell (ESC) lines without the need of genetic manipulation. We thus screened a combinatorial peptide library and identified a..
View full abstractGrants
Awarded by Telethon Foundation
Awarded by Swedish Research Council
Awarded by FIRB
Funding Acknowledgements
We are grateful to the members of the Stem Cell Fate Laboratory and the Arenas laboratory for helpful discussion. This work was supported by the Telethon Foundation to G. M. (grants GGP05112, GGP08120) and S. D. F. (GGP08062), Associazione Italiana Ricerca sul Cancro (AIRC) to G. M. and S. D. F.; and by grants from the European Union (Eurostemcell), the Swedish Research Council (VR2008), Norwegian Research Council and Karolinska Institutet, to E. A. M. R. is funded by a FIRB (project, n<SUP>o</SUP> RBNE03PX83_005). C. L. P. was supported by a Human Frontiers Science Program Long-term Fellowship and a National Health and Medical Research Council, Australia Career Development Award. D. R. was supported by a by a grant form the Portuguese Praxis XXI program of the Fundacao para a Ciencia e a Tecnologia.