Journal article
Adverse effect of the chitinolytic enzyme PjCHI-1 in transgenic tomato on egg mass production and embryonic development of Meloidogyne incognita
Y-L Chan, D Cai, PWJ Taylor, M-T Chan, KW Yeh
PLANT PATHOLOGY | WILEY-BLACKWELL | Published : 2010
Abstract
A novel chitinase gene (PjCHI-1) isolated from Paecilomyces javanicus, a non-nematophagous fungus, and driven by a CaMV35S promoter, was delivered into CLN2468D, a heat-tolerant cultivar of tomato (Solanum lycopersicum). T₁ tomato plants exhibited high endochitinase activity and reduced numbers of eggs and egg masses when infected with the root-knot nematode (RKN) Meloidogyne incognita. The eggs found in transgenic tomato had lower shell chitin contents than eggs collected from control plants. Egg masses from transgenic plants exhibited higher chitinase activity than those from control plants. Moreover, only 30% of eggs from transgenic plants were able to develop to the multi-cell/J1 stage, ..
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Awarded by National Science and Technology Program for Agricultural Biotechnology, Taiwan
Funding Acknowledgements
This work is part of the TW-SOL project (International Solanaceae Genome Project - Taiwan) and was supported by grants from Academia Sinica to M-T C and the National Science and Technology Program for Agricultural Biotechnology [numbers 95AS-621-ST-a1(46), 96AS-121-ST-a2 (13) and 97AS-121-ST-a3 (13)], Taiwan, to KWY. The authors thank DAAD and Taiwan NSC for providing travel grants (PPP-Taiwan) to Kiel University, Germany, and also Dr Samson C. S. Tsou, the former director of AVRDC-The World Vegetable Center, Tainan, Taiwan, for kind suggestions on the project.