Tissue-specific interplay between copper uptake and efflux in Drosophila
Tim Binks, Jessica Charlotte Lye, James Camakaris, Richard Burke
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY | SPRINGER | Published : 2010
The vinegar fly Drosophila melanogaster is proving to be an excellent system to study the in vivo regulation of the essential metal copper. The Ctr1A/B and DmATP7 copper transport proteins have well-established roles in Drosophila copper uptake and efflux, respectively. Both Ctr1A and DmATP7 are essential genes, whereas Ctr1B mutants are viable but die in excess or depleted copper conditions. Less is known about the tissue-specific requirements for these three genes and how they interact to maintain copper homeostasis in different cell types. Here, we use targeted overexpression and suppression of each gene to examine these questions in vivo. We find that in the epidermal cells that form the..View full abstract
This research was supported by an Australian Research Council Discovery Grant and a National Health and Medical Research Council Project Grant. We thank the Vienna Drosophila RNAi Center, Konrad Basler (Zurich University) and Walter Schaffner (Zurich University) for fly stocks.