Journal article

Brain uptake of diazepam and phenytoin in a genetic animal model of absence epilepsy

Joseph A Nicolazzo, Jessica A Steuten, Susan A Charman, Nerida Taylor, Philip J Davies, Steven Petrou

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | WILEY-BLACKWELL | Published : 2010

Abstract

1. Although many studies have assessed changes to brain uptake of anti-epileptic drugs (AEDs) in chemically and electrically induced seizure models, there are limited data available on changes to brain uptake of AEDs in spontaneous seizure animal models, such as genetic absence epilepsy. 2. In the present study, the brain uptake of diazepam and phenytoin was assessed in a genetic mouse model of absence seizures harbouring a human GABA(A) receptor gamma2-subunit gene GABRG2 mutation (R43Q) and results were compared with those obtained during acute seizures induced by subcutaneous administration of pentylenetetrazole (PTZ; 90 mg/kg). Diazepam and phenytoin were administered intraperitoneally a..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by National Institutes of Health National Institute of Neurological Disorders and Stroke


Awarded by NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE


Funding Acknowledgements

This work was supported, in part, by a National Health and Medical Research Council of Australia programme grant (400121; to SP) and National Institutes of Health National Institute of Neurological Disorders and Stroke (NS046378; to SP). SP served as a paid consultant for Bionomics Limited (Thebarton, SA, Australia), which holds the intellectual property surrounding this work. None of the other authors has any conflict of interest to disclose.