Journal article
Gene identification and proteomic analysis of the esterases of the cotton bollworm, Helicoverpa armigera
MG Teese, PM Campbell, C Scott, KHJ Gordon, A Southon, D Hovan, C Robin, RJ Russell, JG Oakeshott
Insect Biochemistry and Molecular Biology | Published : 2010
Abstract
Some of the resistance of Helicoverpa armigera to conventional insecticides such as organophosphates and synthetic pyrethroids appears to be due to metabolic detoxification by carboxylesterases. To investigate the H. armigera carboxyl/cholinesterases, we created a data set of 39 putative paralogous H. armigera carboxyl/cholinesterase sequences from cDNA libraries and other sources. Phylogenetic analysis revealed a close relationship between these sequences and 70 carboxyl/cholinesterases from the recently sequenced genome of the silkworm, Bombyx mori, including several conserved clades of non-catalytic proteins. A juvenile hormone esterase candidate from H. armigera was identified, and B. mo..
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Funding Acknowledgements
We thank Dr Alagacone Sriskantha and Anh Cao at CSIRO for excellent technical assistance in sequencing the cDNA clones, and Dr. Rod Mahon for providing the larvae used in the proteomic experiments. We also thank Drs Alan Devonshire, Bronwyn Campbell, Lisa Bird, David Tattersall, Peter East (CSIRO), Charles Claudianos (The University of Queensland), Takahiro Shiotsuki (NIAS, Japan), David Heckel (Max Planck Institute for Chemical Ecology, Germany), Keshav Kranthi (Central Institute for Cotton Research, Nagpur, India) and Yidong Wu (Nanjing Agricultural University, China) for helpful discussions. This work was supported by Orica Australia.