Journal article
Retinal metabolic state of the proline-23-histidine rat model of retinitis pigmentosa
ML Acosta, YS Shin, S Ready, EL Fletcher, DL Christie, M Kalloniatis
American Journal of Physiology Cell Physiology | AMER PHYSIOLOGICAL SOC | Published : 2010
Abstract
We determined the metabolic changes that precede cell death in the dystrophic proline-23-histidine (P23H) line 3 (P23H-3) rat retina compared with the normal Sprague-Dawley (SD) rat retina. Metabolite levels and metabolic enzymes were analyzed early in development and during the early stages of degeneration in the P23H-3 retina. Control and degenerating retinas showed an age-dependent change in metabolite levels and enzymatic activity, particularly around the time when phototransduction was activated. However, lactate dehydrogenase (LDH) activity was significantly higher in P23H-3 than SD retina before the onset of photoreceptor death. The creatine/phosphocreatine system did not contribute t..
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Awarded by Health Research Council
Funding Acknowledgements
This work was supported, in part, by an Auckland Medical Research Foundation grant, Health Research Council Grant 05/247, a grant from Retina Australia, a professorship funded by the Robert G. Leitl estate (M. Kalloniatis), and the University of Auckland Staff Research Fund (M. Kalloniatis and M. L. Acosta).