Journal article
Antifibrotic activity of bevacizumab on human tenon's fibroblasts in vitro
EC O'Neill, Q Qin, NJ van Bergen, PP Connell, S Vasudevan, MA Coote, IA Trounce, TTL Wong, JG Crowston
Investigative Ophthalmology and Visual Science | Published : 2010
DOI: 10.1167/iovs.10-5669
Abstract
PURPOSE. To evaluate the effect of the anti-VEGF-A monoclonal antibody bevacizumab on primary human Tenon's capsule fibroblasts (HTFs) in an in vitro model of wound healing. METHODS. Fibroblasts were cultured in RPMI media, and bevacizumab was administered at a concentration ranging from 0.25 to 12.5 mg/mL. Fibroblast viability and cell death were assessed using the MTT colorimetric assay, lactate dehydrogenase assay, BrdU assay, and live/dead assay. Fibroblast contractility was assessed in floating collagen gels. Morphologic changes were assessed by transmission electron microscopy. Antifibrosis activities were compared with 5-fluorouracil. RESULTS. Bevacizumab induced a significant dose-re..
View full abstract