Saliva-Derived DNA Performs Well in Large-Scale, High-Density Single-Nucleotide Polymorphism Microarray Studies
Melanie Bahlo, Jim Stankovich, Patrick Danoy, Peter F Hickey, Bruce V Taylor, Sharon R Browning, Matthew A Brown, Justin P Rubio
Cancer Epidemiology, Biomarkers & Prevention | AMER ASSOC CANCER RESEARCH | Published : 2010
As of June 2009, 361 genome-wide association studies (GWAS) had been referenced by the HuGE database. GWAS require DNA from many thousands of individuals, relying on suitable DNA collections. We recently performed a multiple sclerosis (MS) GWAS where a substantial component of the cases (24%) had DNA derived from saliva. Genotyping was done on the Illumina genotyping platform using the Infinium Hap370CNV DUO microarray. Additionally, we genotyped 10 individuals in duplicate using both saliva- and blood-derived DNA. The performance of blood- versus saliva-derived DNA was compared using genotyping call rate, which reflects both the quantity and quality of genotyping per sample and the "GCScore..View full abstract
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Awarded by Australian Research Council
M. Bahlo is supported by Career Development Award from The National Health and Medical Research Council of Australia (NHMRC). M. A. Brown is an NHMRC Principal Research Fellow. H. Butzkueven ( AN-Zgene Consortium), is an NHMRC Peter Doherty Postdoctoral Fellow. J. Field is an MSRA Postdoctoral Fellow. M. B. Cox is supported by a grant from the John Hunter Hospital Charitable Trust Fund and a special grant from Macquarie Bank. This work was supported by the Ian Potter Foundation, the Australian Cancer Research Foundation, MSRA, the John T. Reid Charitable Trusts, the Trish MS Research Foundation, and the Australian Research Council, under the Linkage Projects Scheme (LP0776744).