Molecular detection of Legionella: moving on from mip
Stacey FY Yong, Shin Hwa Tan, Joanne Wee, Jing Jhi Tee, Fiona M Sansom, Hayley J Newton, Elizabeth L Hartland
Frontiers in Microbiology | FRONTIERS RESEARCH FOUNDATION | Published : 2010
The detection of Legionella pneumophila in environmental and clinical samples is frequently performed by PCR amplification of the mip and/or 16S rRNA genes. Combined with DNA sequencing, these two genetic loci can be used to distinguish different species of Legionella and identify L. pneumophila. However, the recent Legionella genome sequences have opened up hundreds of possibilities for the development of new molecular targets for detection and diagnosis. Ongoing comparative genomics has the potential to fine tune the identification of Legionella species and serogroups by combining specific and general genetic targets. For example, the coincident detection of LPS biosynthesis genes and viru..View full abstract
This work was supported by grants awarded to Elizabeth L. Hartland from the Australian National Health and Medical Research Council (NHMRC) and Stacey F. Y. Yong from the Ministry of Science, Technology and Innovation, Malaysia. Elizabeth L. Hartland is supported by an Australian Research Council Future Fellowship. Hayley J. Newton and Fiona M. Sansom are supported by NHMRC training fellowships.