General method for selective labelling of double-chain cysteine-rich peptides with a lanthanide chelate via solid-phase synthesis
Fazel Shabanpoor, Frances Separovic, John D Wade
JOURNAL OF PEPTIDE SCIENCE | WILEY | Published : 2011
The use of lanthanides in preference to radioisotopes as probes for various biological assays has gained enormous popularity. The introduction of lanthanide chelates to peptides/proteins can be carried out either in solution using a commercially available labelling kit or by solid-phase peptide synthesis using an appropriate lanthanide chelate. Herein, a detailed protocol for the latter is provided for the labelling of peptides or small proteins with diethylenetriamine-N, N, N″, N″-tetra-tert-butyl acetate-N'-acetic acid (DTPA) chelate or other similar chelates on a solid support using a chimeric insulin-like peptide composed of human insulin-like peptide 5 (INSL5) A-chain and relaxin-3 B-ch..View full abstract
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Awarded by NHMRC
Fazel Shabanpoor is a recipient of David Hay Postgraduate Write-up award from the University of Melbourne. This work was supported by NHMRC Project grants (#350245 and 509048) to JDW.