Journal article
Nonsense mediated decay resistant mutations are a source of expressed mutant proteins in colon cancer cell lines with microsatellite instability
DS Williams, MJ Bird, RN Jorissen, YL Yu, F Walker, HH Zhang, EC Nice, AW Burgess
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2010
Abstract
Background: Frameshift mutations in microsatellite instability high (MSI-High) colorectal cancers are a potential source of targetable neo-antigens. Many nonsense transcripts are subject to rapid degradation due to nonsense-mediated decay (NMD), but nonsense transcripts with a cMS in the last exon or near the last exon-exon junction have intrinsic resistance to nonsense-mediated decay (NMD). NMD-resistant transcripts are therefore a likely source of expressed mutant proteins in MSI-High tumours. Methods: Using antibodies to the conserved N-termini of predicted mutant proteins, we analysed MSI-High colorectal cancer cell lines for examples of naturally expressed mutant proteins arising from f..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This project was partially funded by NHMRC Program Grant number 487922. Funding support for this work was also provided by a CASS (Contributing to Australian Scholarship & Science) Foundation Science and Medicine Grant (DSW, AWB, ECN), the Cancer Council Victoria (EN) and a Royal College of Pathologists of Australasia Kitamura/Kanematsu Memorial Award and Technical Assistance Grant (DSW). This work was also supported by funds from the Operational Infrastructure Support Program provided by the Victorian Government, Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.