Isolation, identification and biological activity of gastrin-releasing peptide 1-46 (oGRP(1-46)), the primary GRP gene-derived peptide product of the pregnant ovine endometrium
AS Giraud, C Dumesny, JC Whitley, LM Parker, I Jennings, B Kemp, TW Moody, V Sancho, RT Jensen, A Shulkes
PEPTIDES | ELSEVIER SCIENCE INC | Published : 2010
We have previously demonstrated that pregnant ovine endometrium expresses the gastrin-releasing peptide (GRP) gene at a high level following conceptus implantation. Here we report the isolation, characterization and biological activity of ovine GRP 1-46, the primary product of this gene in the pregnant endometrium. Full thickness 125-140-day pregnant sheep uterus (term is 145 day) was homogenized in 80% acetonitrile/2% trifluoroacetic acid (1:7 ACN/TFA), concentrated on reverse-phase C18 cartridges and chromatographed successively on gel filtration (Sephadex G-50) and reverse-phase HPLC (C18 muBondapak). Purification was monitored by RIA. Purified GRP peptide was analysed by mass spectrometr..View full abstract
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
We thank the late Professor John Walsh and the late Ms Helen Wong (Department of Medicine, UCLA, Los Angeles, CA) for donating antisera 8684. This work was supported by projects grants from the National Health and Medical Research Council of Australia and intramural funds of NIDDK, NIH.