Journal article

Isolation, identification and biological activity of gastrin-releasing peptide 1-46 (oGRP(1-46)), the primary GRP gene-derived peptide product of the pregnant ovine endometrium

AS Giraud, C Dumesny, JC Whitley, LM Parker, I Jennings, B Kemp, TW Moody, V Sancho, RT Jensen, A Shulkes

PEPTIDES | ELSEVIER SCIENCE INC | Published : 2010

Abstract

We have previously demonstrated that pregnant ovine endometrium expresses the gastrin-releasing peptide (GRP) gene at a high level following conceptus implantation. Here we report the isolation, characterization and biological activity of ovine GRP 1-46, the primary product of this gene in the pregnant endometrium. Full thickness 125-140-day pregnant sheep uterus (term is 145 day) was homogenized in 80% acetonitrile/2% trifluoroacetic acid (1:7 ACN/TFA), concentrated on reverse-phase C18 cartridges and chromatographed successively on gel filtration (Sephadex G-50) and reverse-phase HPLC (C18 muBondapak). Purification was monitored by RIA. Purified GRP peptide was analysed by mass spectrometr..

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