Journal article
Comparison of two Mycoplasma genitalium real-time PCR detection methodologies
J Twin, N Taylor, SM Garland, JS Hocking, J Walker, CS Bradshaw, CK Fairley, SN Tabrizi
Journal of Clinical Microbiology | Published : 2011
DOI: 10.1128/JCM.02328-10
Abstract
Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399). Copyright © 2011, American Society for Microbiology. All Rights Reserved.