Journal article
Comparison of Two Mycoplasma genitalium Real-Time PCR Detection Methodologies
Jimmy Twin, Nicole Taylor, Suzanne M Garland, Jane S Hocking, Jennifer Walker, Catriona S Bradshaw, Christopher K Fairley, Sepehr N Tabrizi
JOURNAL OF CLINICAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2011
DOI: 10.1128/JCM.02328-10
Abstract
Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).