Journal article

Plasmodium falciparum: Genetic and immunogenic characterisation of the rhoptry neck protein PfRON4

Belinda J Morahan, Georgina B Sallmann, Robert Huestis, Valentina Dubljevic, Karena L Waller



The Apicomplexan parasites Toxoplasma and Plasmodium, respectively, cause toxoplasmosis and malaria in humans and although they invade different host cells they share largely conserved invasion mechanisms. Plasmodium falciparum merozoite invasion of red blood cells results from a series of co-ordinated events that comprise attachment of the merozoite, its re-orientation, release of the contents of the invasion-related apical organelles (the rhoptries and micronemes) followed by active propulsion of the merozoite into the cell via an actin-myosin motor. During this process, a tight junction between the parasite and red blood cell plasma membranes is formed and recent studies have identified r..

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University of Melbourne Researchers


Funding Acknowledgements

We thank Robin Anders and Michael Foley for supplying the anti-PfAMA1 monoclonal 1F9 antibody and for critical discussions during the course of this investigation. We thank Dave Richard and Alan Cowman for supplying the anti-PfRON4 monoclonal antibody. We thank MR4 (Malaria Research and Reference Reagent Resource, ATCC) for providing the anti-GRP(BiP) and anti-EBA-175 antibodies (both contributed by John H. Adams) and Sally Troy for her technical assistance. K.L.W. was supported by an NHMRC Howard Florey Centenary Research Fellowship.