Journal article
Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green i dye
MLJ Lay, RM Lucas, M Ratnamohan, J Taylor, AL Ponsonby, DE Dwyer
Virology Journal | BMC | Published : 2010
Open access
Abstract
Background. Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of virus activity. Two separate real-time quantitative polymerase chain reaction (QPCR) assays using SYBR Green I dye and a single quantification standard containing two EBV genes, Epstein-Barr nuclear antigen-1 (EBNA-1) and BamHI fragment H rightward open reading frame-1 (BHRF-1), were developed to detect and measure absolute EBV DNA load in patients with various EBV-associated diseases. EBV DNA loads and viral capsid antigen (VCA) IgG antibody..
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Awarded by National Multiple Sclerosis Society
Funding Acknowledgements
The Ausimmune Investigator Group includes C Chapman, A Coulthard, K Dear, T Dwyer, T Kilpatrick, R Lucas, T McMichael, MP Pender, A-L Ponsonby, B Taylor, P Valery, I van der Mei and D Williams. The Ausimmune Study is funded by the National Multiple Sclerosis Society of the USA, the National Health & Medical Research Council (Project Grant 316901) and Multiple Sclerosis Research Australia. We also acknowledge the work of the Ausimmune Study research nurses who undertook sample collection: S Agland, B Alexander, M Davis, Z Dunlop, A Wright, R Scott, J Selvidge, M Steele, K Turner, B Wood and the study project officers, H Rodgers and C Jozwick. Clinical samples were kindly provided by N Gilroy, D Gottlieb, P Ferguson, F Kwok and I Kay. We would also like to thank B Wang of the Westmead Millennium Institute for assisting with the cloning work, B O'Toole for statistical analyses, D Patel for assistance with the serology and C Toi for laboratory guidance and review of the manuscript.