Journal article

Memory precursor phenotype of CD8( ) T cells reflects early antigenic experience rather than memory numbers in a model of localized acute influenza infection

Hayley A Croom, Alice E Denton, Sophie A Valkenburg, Natasha G Swan, Matthew R Olson, Stephen J Turner, Peter C Doherty, Katherine Kedzierska

EUROPEAN JOURNAL OF IMMUNOLOGY | WILEY-BLACKWELL | Published : 2011

Abstract

The mechanistic basis of memory T-cell development is poorly defined. Phenotypic markers that define precursors at effector stages have been characterized for acute systemic infections with high antigen load. We asked whether such markers can identify memory precursors from early effectors (d6) to late memory (>d500) for two immunodominant CD8(+) responses during the course of a localized low-load influenza infection in mice. CD8(+) T cells stained with the D(b) NP(366) and D(b) PA(224) tetramers were characterized as IL-7Rα(hi) , IL-7Rα(hi) CD62L(hi) or IL-7Rα(hi) KLRG1(lo) . While the D(b) NP(366) - and D(b) PA(224) -specific responses were comparable in size, decay kinetics and memory pre..

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Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by NIH


Awarded by NHMRC


Funding Acknowledgements

The authors thank Dr. La Gruta for review of the manuscript and Ken Field for FACS sorting. This work was supported by Australian National Health and Medical Research Council (NHMRC) Project Grants to K. K. (AI454312), P. C. D. (AI454595), an NHMRC Program Grant APP567122 (to PCD and SJT) and NIH grant AI170251. K. K. is an NHMRC RD Wright Fellow; S. J. T. is a Pfizer Senior Research Fellow. H. A. C. and A. E. D. are recipients of the NHMRC Biomedical Postgraduate Scholarship; S.A.V. is a recipient of the Australian Postgraduate Award.