Journal article

Study of the aminopeptidase N gene family in the lepidopterans Ostrinia nubilalis (Hubner) and Bombyx mori (L.): Sequences, mapping and expression

Cristina M Crava, Yolanda Bel, Siu Fai Lee, Barbara Manachini, David G Heckel, Baltasar Escriche

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2010

Abstract

Aminopeptidases N (APNs) are a class of ectoenzymes present in lepidopteran larvae midguts, involved in the Bacillus thuringiensis (Bt) toxins mode of action. In the present work, seven aminopeptidases have been cloned from the midgut of Ostrinia nubilalis, the major Lepidopteran corn pest in the temperate climates. Six sequences were identified as APNs because of the presence of the HEXXH(X)18E and GAMEN motifs, as well as the signal peptide and the GPI-anchor sequences. The remaining sequence did not contain the two cellular targeting signals, indicating it belonged to the puromycin-sensitive aminopeptidase (PSA) family. An in silico analysis allowed us to find orthologous sequences in Bom..

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University of Melbourne Researchers

Grants

Awarded by EU


Awarded by Generalitat Valenciana


Awarded by Spanish Ministerio de Educacion y Ciencia


Awarded by University of Valencia


Funding Acknowledgements

The present project was funded by EU Fifth Framework Project "ProBenBt" (QLK3-CT-2002-01969). Additional support was provided by the Generalitat Valenciana (GVARVIV2007-090 and ACOMP/2009/313), by the Spanish Ministerio de Educacion y Ciencia (AGL2006-11914), by European FEDER funds, by CESAR, a Special Research Centre of the Australian Research Council, and by the Max-Planck-Gesellschaft. M.C. Crava was funded by a fellowship V Segles from the University of Valencia (UV-BVS-07-2083), Y. Bel was financed by the Spanish Ministerio de Educacion y Ciencia (AGL2006-11914), and S.F. Lee was supported by the University of Melbourne and the Max-Planck-Gelsellschaft. Thanks to Prof. Dr. Ingolf Schuphan and Dr. Christiane Saeglitz of RWTH University, Aachen, for support of the mapping project, and to Dr. Heiko Vogel and Domenica Schnabelrauch of MPI Chemical Ecology for EST sequencing.