Journal article

Stimulation of Activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells

Caitlin E Filby, Robert Williamson, Peter van Kooy, Alice Pebay, Mirella Dottori, Ngaire J Elwood, Faten Zaibak



INTRODUCTION: Unrestricted somatic stem cells (USSC) derived from umbilical cord blood are an attractive alternative to human embryonic stem cells (hESC) for cellular therapy. USSC are capable of forming cells representative of all three germ line layers. The aim of this study was to determine the potential of USSC to form definitive endoderm following induction with Activin A, a protein known to specify definitive endoderm formation of hESC. METHODS: USSC were cultured for (1) three days with or without 100 ng/ml Activin A in either serum-free, low-serum or serum-containing media, (2) three days with or without 100 ng/ml Activin A in combination with 10 ng/ml FGF4 in pre-induction medium, o..

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Funding Acknowledgements

We wish to thank Professor Stuart Schreiber for the kind gift of the IDE1/2 molecules, Jessie Leung for assistance with hESC cultures and Kate Loveland, Julia Young and Jessica Van Gent for the kind gift of the ACVR antibodies and assistance with ACVR immunohistochemistry. We would also like to acknowledge the Justin Lowndes Cystic Fibrosis Research Bequest, the Australian Cystic Fibrosis Research Trust and the CASS (Contributing to Australian Scholarship and Science) Foundation for their support.