Journal article
Structural dynamics of a lytic peptide interacting with a supported lipid bilayer
AC Rapson, MA Hossain, JD Wade, EC Nice, TA Smith, AHA Clayton, ML Gee
Biophysical Journal | Published : 2011
Abstract
The interaction of a melittin mutant with a 1,2-dipalmitoyl-sn-glycero-3- phosphatidylcholine (DPPC)-supported lipid bilayer was studied with the use of time-resolved evanescent wave-induced fluorescence spectroscopy (TREWIFS) and evanescent wave-induced time-resolved fluorescence anisotropy measurements (EW-TRAMs). The mutant peptide was labeled at position K14 with AlexaFluor 430 and retained the lytic activity characteristic of native melittin. The fluorescence decay kinetics of the conjugate was found to be biexponential with a short-lived component, τ1, due to photoinduced electron transfer between AlexaFluor 430 and proximal side chains within or between the peptides. The longer-lived ..
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Awarded by Australian Research Council
Funding Acknowledgements
A. C., E. N., and M. G. gratefully acknowledge the Australian Research Council for financial support for this project in the form of a Discovery grant No. DP0557718.